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Image Search Results
Journal: Cellular and Molecular Immunology
Article Title: Cyclic adenosine monophosphate involvement in low-dose cyclophosphamide-reversed immune evasion in a mouse lymphoma model
doi: 10.1038/cmi.2012.34
Figure Lengend Snippet: Changes in percentages of CD4+CD25+ lymphocytes and FOXP3 expression levels. (a) Lymphocytes were harvested at the indicated time points and dual-stained with CD25 and CD4 mAbs. The percentages of Tregs in the total lymphocytes on different days are shown. Non-specific antibody labeling was determined using isotype-matched negative control antibodies. (b) The ratio of Treg/CD4+ cells from Figure 2a is shown as a bar graph. The difference between the BALB/c and tumor groups was significant (P=0.003). The ratio of Treg/CD4+ cells in the tumor and CY8 groups was significantly different (the tumor group vs. the CY8 group, P=0.0004), and levels in the CY14 group slightly exceeded those in the tumor group (tumor group vs. CY14 group, P=0.4056). (c) Equivalent numbers of the lymphocytes at the indicated time points were harvested, and total RNA was extracted for real-time PCR. All data are representative of three independent experiments and expressed as the mean±s.d. The FOXP3 mRNA expression of specified groups significantly differed (tumor group vs. BALB/c group, *P=0.0164; CY8 group vs. tumor group, ***P<0.001). (d, e) Equivalent numbers of lymphocytes were harvested at the indicated time points with or without A20 cells and CY, and total protein was extracted for western blot analyses. d shows the intensity of the FOXP3 band normalized to the β-actin band. e shows the CY treatment transiently reduced the protein expression of FOXP3. The changes in protein levels were consistent with the mRNA changes that are shown in c. CY, cyclophosphamide; mAb, monoclonal antibody; PCR, polymerase chain reaction; Treg, regulatory T cell.
Article Snippet: A
Techniques: Expressing, Staining, Antibody Labeling, Negative Control, Real-time Polymerase Chain Reaction, Western Blot, Polymerase Chain Reaction